Frequency of beta toxin and phisa3 genes in Staphylococcus aureus isolates collected from multiple sclerosis (MS) patients and healthy nasal carriers in Tabriz

Abstract

The incidence of Staphylococcus aureus infection is alarming as a significant public health problem. This bacterium contributes to the development of multiple sclerosis (MS) disorder in young and adult individuals. This research aimed to determine the frequency of hlb, int of phi3, and mecA genes in 130 S. aureus isolates collected from MS-patients and healthy nasal carriers with PCR method in Tabriz. The nuc gene was used for confirming S. aureus identification. Also antibiotic susceptibility patterns was conducted employing antibiotics with disk diffusion agar method in two groups of study. Material and Methods: Four hundred nasal swabs were collected from MS patients (140) and healthy nasal carriers (260). The isolates were identified by phenotypic tests including catalase, coagulase, growth on mannitol salt agar, and DNase activity. Antibiotic susceptibility was conducted by disk diffusion agar method. Besides, PCR was done to the detection of hlb, int of phi3, nuc, and mecA genes. One hundred thirty isolates were identified as S. aureus from MS patients (65 isolates) and healthy nasal carriers (65 isolates). Results: The highest resistance rate was to erythromycin in MS patients (47.7%) and healthy nasal carriers (55.4%) isolates. All isolates of healthy nasal carriers and 64 (98.5%) isolates of MS patients were susceptible to linezolid. The percentage of isolates containing the hlb, int of phi3, and mecA in MS patients was 15.38%, 41.53%, 18.46% and in healthy nasal carriers 6.15%, 18.46%, 21.5%, respectively.