Study of the effect DHA fatty acid on the expression of immune checkpoint molecules and their related miRNAs in gastric cancer cell line

Abstract

The aim of this study was to investigate the expression of these genes and miRNAs that control them in hypoxic conditions. Material and Methods: MKN-45 gastric cancer cell line was cultured in the relevant culture medium and after treatment with DHA and DTX, RNA was extracted. Then cDNA was synthesized. Finally, changes in the expression of B7-H3, B7-H4, PD-L1 genes and their control miRNAs were determined by qRT-PCR. Results: QRT-PCR test changes in the expression of B7-H3, B7-H4, PD-L1 genes and miRs controlling them in treatment with DHA and DTX conditions in terms of gene expression B7-H3, B7-H4, PD-L1 showed a significant decreased, while expression of miR-143 significant increased. The expression of miR-155 did not decrease significantly.