The association of the phylogenetic typing of the Klebsiella pnumoniae isolates with antibiotic resistance

Abstract

Klebsiella pneumonia (K. pneumonia) causes about one-third of all Gram-negative infections, is highly resistant; and was historically subdivided into KpI, KpII, and KpIII phylogroups. The study aimed to investigate the distribution of phylogenetic groups and the relationship between antibiotic resistance patterns and phylogenetic groups. Materials and Methods: We collected K. pneumonia isolates from Imam Reza Hospital, Tabriz, Iran from 2018 until 2020. Antimicrobial susceptibility testing was performed by disk diffusion agar and phylogenetic groups were examined using gyrA restriction fragment length polymorphism (RFLP) and parC PCR methods. Results: A total of 100 K. pneumonia isolates were obtained from clinical specimens. The patients were aged 1 to 91 years and composed of 47 males and 53 females. The highest sensitivity was found to amikacin 66% and followed by imipenem 50%. The three phylogenetically groups by RFLP-PCR method was found in K. pneumonia, 96% (96 cases) as KpI group, 3% (3 cases) KpII, and 1% (1 case) KpIII. The highest resistance was observed in KpI group, followed by KpII and KpIII groups (Pv = 0.01).