| dc.description.abstract | Due to the increasing prevalence of CRC and the development of drug resistance especially to
oxaliplatin, it is necessary to investigate the development of resistance to drugs used in
conventional treatment methods. Therefore, in this study expression analysis of the genes involved
in oxaliplatin resistance in colorectal cell line were investigated. Purpose: The aim of this project
was to evaluate the expression of the genes involved in oxaliplatin resistance. Procedure: Both
oxaliplatin resistant Human colorectal Caco2 and regular Caco2 cell lines were grown as
monolayer in RPIM 1640 medium supplemented with 10% of heat-inactivated FBS and cultured
at 37˚C in a humidified atmosphere of 5% CO2. Subsequently both cell lines were treated with
different concentrations of oxaliplatin. After 48 hours, total RNA of cells was extracted and the
synthesize of cDNA was completed through a reverse transcriptase. Finally, expression analysis
of some of the involved genes in oxaliplatin resistance were done in all of the groups using Realtime
PCR method. All the results were analysed using ΔΔCt method. Findings: The results of
Real-time PCR indicate that the expression of the genes including ATP7 ،ERCC1 ،AKT1 ،mTOR ،
BCL-XL ،GpX4 ،NRF2 ،Vimentin ،SNAIL1 and Twist1 after oxaliplatin treatment were increased
in resistant cell line compared to the sensitive cell line, while the expression of E-cadherin was
decreased after oxaliplatin treatment in resistance cell line compared to the sensitive cell
line.Conclusion: According to the results of this study, these genes can be used as drug targets in
the destruction of resistant cells suggesting that the use of appropriate strategies to inhibit the genes
including ATP7, ERCC1, AKT1, mTOR, BCL-XL, GpX4, NRF2, Vimentin, SNAIL1 and Twist1
and the enhancement of the expression of E-cadherin may be effective to reduce the resistancy in
colorectal cell line. | en_US |
