The evaluation of rs2228145 polymorphism of IL-6R gene in patients with diabetic nephropathy

Abstract

The aim of this study was to determine the relationship between rs7529229 and rs2228145 polymorphisms of IL-6R gene with the incidence of DN disease in this study. Materials & methods Blood samples were collected from 113 diabetic patients including 56 patients with nephropathy and 57 patients without nephropathy and 50 healthy individuals. After DNA extraction, all samples were sequenced with specific primers by (Restriction fragment length polymorphism-Polymerase Chain Reaction (RFLP-PCR) method. The obtained results were analyzed using SPSS software version 23. Results Evaluation of IL-6R gene polymorphisms in the incidence of nephropathy among type 2 diabetic patients showed that the presence of rs2228145 IL-6R gene polymorphism is associated with the incidence of nephropathy in diabetic patients compared with healthy individuals (P = 0.022). However, the frequency distribution of rs7529229 polymorphism in DN subjects was not statistically significant either in comparison with diabetic patients or in healthy individuals.