The study of the levels of oncostatin M and its receptor in granulosa cells and follicular fluid of women with polycystic ovarian syndrome and investigation of human recombinant oncostatin M effects on granulosa cells in vitro

Abstract

The involvement of Oncostatin M (OSM), a recently identified adipokine, in ovarian function is unknown. Therefore, we investigated the association of OSM signaling pathway with ovarian functions and PCOS pathogenesis. Materials and Methods: In the in-vivo experiment, 30 PCOS and 30 healthy women who underwent the ICSI procedure were enrolled. The lipid, carbohydrate, and hormonal profiles, as well as OSM and OSMR levels, were evaluated in the follicular fluid (FF). Moreover, the expression of insulin receptor substrates (IRS1 and IRS2), OSM, OSMR, suppressor of cytokine signaling 3 (SOCS3), and androgen receptor (AR) genes were analyzed in the isolated cumulus cells (CCs) and also after treatment with recombinant OSM. Results: Follicular concentrations of OSM and OSMR were significantly lower in PCOS compared to control women (p<0.001) and were positively correlated with the oocyte maturation and fertilization rates in the PCOS group. Furthermore, the SOCS3 expression was upregulated in PCOS patients compared to the controls (p=0.043). The treatment of cells with recombinant OSM significantly increased SOCS3, OSMR, IRS-1, and -2 expression and decreased AR expression.