Evaluation of prophylactic effects of Amantadine and Vitamin C on neuropathic pain induced by Cisplatin in mice

Abstract

IntroductionIn the process of neuropathy induced by anticancer drugs, several mechanisms have been mentioned such as activation of the oxidative stress system, increasing the activity of N-methyl-D-aspartate(NMDA) receptors, and serotonin receptors. Goal: The aim of the current study was to evaluate the preventive effects of Amantadine and Vitamin C on Cisplatin-induced neuropathic pain that was the result of cisplatin in the (spurious) mice. Materials and methods: Seventy-two male mice in a weight range of 25 to 35 g were randomly divided into 9 equal groups and underwent Amantadine and Vitamin C for 25 days. Different doses of Amantadine and Vitamin C were injected three days before injection of Cisplatin (5 mg/kg,IP ) at 4th day. Then injection of Amantadine and Vitamin C continued for 3 days. After that we have 3 days for rest. Finally we performed hot plate test. This 7 day cycle repeated 3 times for all groups. At day 25, blood samples were collected for Malondialdehyde )MDA( and و Total Antioxidant Capacity (TAC) Test. Result: The results was showed a meaningful (p-value<0.05) increase in reaction to the pain after injection of these doses (20 mg/kg, IP) of amantadine and Vitamin C (100 mg/kg, IP) and also was observed the meaningful impact of Amantadin ( 5, 10, 20 mg/kg, IP) and Vitamin C( 100 mg/kg, IP) in these doses can decrease MDA level. Also, co-injection of the minimum doses of this drug had a statistically better effect than injection of each of them alone. Conclusion: It is probable that a part of the inhibitory effects of Amantadine and Vitamin C is mediated by the decreasing of Inflamation in CNS.