The effect of intratracheal delivery of Conditioned medium from bone marrow-derived mesenchymal stem cells on the apoptosis in lung tissues of ovalbumin-induced asthmatic male rats

Abstract

Beside to therapeutic effects of stem cells driven by transdifferentiation, it has been shown that the most regenerative capacity of these cells are related to release of different growth factors in a paracrine manner. Therefore, in the current study, the effect of intratracheal delivery of mesenchymal stem cell-derived conditioned media (MSC-CM) on the pathological changes and expression of apoptosis-related genes in lung tissues of ovalbumin-induced asthmatic male rats were examined. Methods: Thirty-two male wistar rats were used in the current study. Eight rats were used randomly for collection MSC-CM. The remaining animals were assigned into 3 experimental groups; control (C); Asthmatic (S) and Asthmatic + MSC-CM (S + MSC-CM). Two weeks post-injection of MSC-CM, pathological changes and the expression of TNF-α and apoptosis -related genes in lung tissue was investigated. Results: Data showed pathological changes in pulmonary tissue of asthmatic groups (S and S + MSC-CM groups) coincided with the up-regulation of TNF-α، Caspase-3،Bax, Bax/Bcl-2 ratio and down regulation of Bcl-2 compared to the control group. Intratracheal delivery of MSC-CM inhibited OVA -induced changes.