Optimization and production of plant stem cell from oryza sativa and extraction of their aqueous and ethanolic extracts and invitro efficacy as in whitening skin

Abstract

Introduction: Over time, the skin is subject to unwanted aging conditions such as dullness and skin blemishes. One of the factors to deal with skin threatening factors is the use of creams and lotions containing natural and biologically active substances, which are often a better choice due to their ease of use and lower risk of side effects. Continuous production of these products in laboratory conditions and optimization of conditions is beneficial due to the ability to control conditions and spend less time. Recently, the use of callus extract of plants, especially rice, in order to observe the anti-aging and lightening effects of the skin in cosmetic products has received much attention. Aim: In the first place, to promote suitable cell lines in rice plants and then to prove the capabilities of rice callus extract on skin melanoma cells (B16F10) and skin brightness. Methods: Rice seeds with certain conditions and by inducing callus formation with different conditions and then extraction by sonicator with aqueous and ethanol solvents, evaluation of extracts by laboratory tests including measurement of phenol and flavonoids, antioxidant properties, total protein And total carbohydrates were performed by standard methods. Also, the presence of protein macromolecules was investigated, and finally cell studies were performed to evaluate the effect of the extracts in terms of skin brightness on the melanoma cell line of mice. Results: After examining the physicochemical properties, the optimal extracts were selected, and by continuing the tests, the effect of rice callus extracts on skin brightness was proven. Conclusion: Our studies showed that rice callus extract with different culture conditions and solvents, compared to control groups, is effective in brightening the skin and can be introduced as a new substance for cosmetic formulations.