The effect of crocin on autophagy-related genes in lung tissue of ovalbumin sensitized mice

Abstract

Therapeutic effects of crocin have been highlighted in inflammatory lung disorders such as asthma. To best of our knowledge, there are few reports indicating the underlying mechanisms related to the application of crocin in asthma. In present study, we evaluated the possible effects of crocin on autophagy-related genes in lung tissue of ovalbumin sensitized mice. Methods: 40 mice were randomly allocated into five groups; Control group (C); Asthmatic group (A); Asthmatic +crocin 25 group (A+cro25), Asthmatic +crocin 50 group (A+cro50) and Asthmatic +crocin 100 group (A+cro100). In the asthmatic groups, mice were exposed to ovalbumin for a period of 32 days. Crocin was injected intraperitoneally (25, 50, and 100 mg/kg) for consecutive 5 days. One day after sensitization, the expression of autophagy related genes was measured along with pathological injuries in the lung tissues. Results: Data showed pathological changes coincided with up-regulation of Becline-1, LC3 and P62 in in lung tissue of asthmatic groups in compared to the control group. Crocin in 50 and 100 mg/kg dosage had potential to inhibit ovalbumin-induced autophagy response while low dose (25 mg/kg dosage) did not yield any beneficial effects.