Expression comparison of NR3C1 in patients with sudden deaf

Abstract

The aim of this study is to investigation of the expression comparison of NR3C1 in patients with sudden deaf. Materials and Methods: In this retrospective study, 80 samples were examined equally in two groups of 40 patients with SSNHL whose disease was confirmed by a specialist and healthy individuals without hearing impairment. The sampling method of this design was easy to access. After selecting the patients, blood samples were collected from the participants (after obtaining informed consent) in the amount of 5 cc of blood. To protect the RNAs, the samples were quickly transferred to -80 ° C. RNA was extracted from the blood using a traysol solution; then, cDNA synthesis for the genes was performed using cDNA synthesis kits. Finally, using Real Time PCR kit and specific primers of the studied genes, the expression of target genes in the samples was evaluated. The data obtained from measuring the expression of these genes were normalized by measuring the expression level of a house keeping gene called GAPDH and then compared. Results: In this study, the mean (standard deviation) age of the two groups of patients with SSNHL and healthy individuals without hearing impairment was 55.48 (±2.3) and 56.80 (±2.6) years old, respectively. In the group of patients with SSNHL, the side of the auditory involvement was left in 18 cases (45.0%) and right in 22 cases (55.0%), respectively. Also, the highest severity of hearing loss was moderate in the group of patients with SSNHL, with 18 cases (45.0%), followed by mild, severe and profound hearing loss of 10 cases (25.0%), 7 cases (17.5%) and 5 cases (12.5%), respectively. The expression level of NR3C1 gene was not significantly different with clinicopathological features and between men and women (P-value>0.05); Also, the expression level of NR3C1 gene was not significantly different between the two groups of patients with SSNHL and healthy individuals without hearing impairment (P-value>0.05).