Evaluation of the effect of administration of troxerutin on morphine dependence in mice

Abstract

Introduction: Chronic morphine use is associated with increased oxidative stress and inflammatory factors. Troxerutin is a natural bioflavonoid and has antioxidant and anti-inflammatory effects. Aim: The effects of troxerutin on morphine dependence in mice Method: Troxerutin was prepared. After examining the composition of the extract, the experiment was performed in six groups of 8 mice, so that one group received eight days of morphine with normal saline as an extract carrier, one group received only normal saline, and one group received troxerutin with normal saline. The other three groups received three different doses of troxerutin along with morphine. The ninth day, withdrawal symptoms were recorded after naloxone injection and blood samples were examined for antioxidant factors. Results: The total withdrawal score in the 50 mg/kg dose of extract group with morphine was significantly lower than morphine-saline group (p<0.001***). The total withdrawal score in the 100 mg/kg dose of extract group with morphine was significantly lower than morphine-saline group (p<0.01**). No significant sedative and locomotion activity reduction was observed in mice after using troxerutin. (all P>0.05). Antioxidant tests did not yield acceptable results due to possible errors in various parts of the process. Conclusion: Troxerutin reduces the symptoms of morphine withdrawal syndrome in a dose-independent manner. The results of antioxidant tests were not acceptable.