Analysis of correlation between glucocorticoid receptor polymorphisms and new-onset diabetes in renal recipients

Abstract

New-onset diabetes after transplantation (NODAT) is one of severe metabolic complications after transplantation. Glucocorticoid receptor is encoded by NR3C1 gene and it seems that polymorphism in this gene leads to sensitivity increase to insulin. Therefore, aim of the study was evaluation of 4 common polymorphism frequencies in NR3C1 gene of NODAT patients and their relationship with diabetes.

Material & methods. Blood samples were collected from 32 diabetic renal-transplanted and 59 non-diabetic renal-transplanted patients. After DNA extraction, samples were amplified via specific primers using PCR method. Data analysis was performed with SPSS 17.0 software.

Results. Results demonstrated that there are no correlation between diabetes incidence and 4 investigated polymorphisms of glucocorticoid receptor gene. Nevertheless, it was determined that diabetic patients’ age were higher than non-diabetic patients and transplant rejection in diabetic patients was reported more than non-diabetic patients.

Conclusion. Based on gathered information in this research, none of inhibitor and resonator polymorphisms had effect on NODAT development. However, further investigations should be performed in higher sample sizes.