The association of single nucleotide polymorphisms of vitamin D receptor gene and vitamin D binding protein gene and serum level of vit D with renal allograft function and survival in kidney transplant recipients

Abstract

The aim of this study was to investigate the relationship between single nucleotide polymorphisms of vitamin D receptor gene (VDR) and vitamin D-binding protein gene (DBP) and serum levels of vitamin D with function and survival in kidney transplant patients. Materials and Methods: Vitamin D levels and other laboratory information and Patients renal function in last 3 years was assessed using serum creatinine level index and GFR calculation according to patients' routine tests (available in the file). For genetic analysis, 2 cc of blood was taken from the subjects. It should be noted that after obtaining the results, individuals were classified based on genotype and kidney function, and also genotyping in 100 healthy individuals (without kidney transplantation) as a control group was performed and compared with the patient group. Results: Compared to healthy individuals with kidney transplant patients, the frequency of G allele in rs7975232 polymorphism is 0.63 times higher in healthy individuals than in kidney transplant patients (p = 0.026). Regarding survival, there was no difference in the frequency of alleles and the distribution of genotypes of VDR polymorphisms, but in rs7040 polymorphism, it was observed that the G allele ratio was significantly lower by 0.27 in patients who had renal transplant rejection. Be (p = 0.002). According to the history of viral infections, the frequency of T allele in rs2228570 polymorphism with a ratio of 2.03 times is significantly lower in infected people (p = 0.030). In summary, the results of the present study show that for kidney transplantation, Apal polymorphism with low G allele frequency, for survival of rs7040 polymorphism with low G allele frequency, and for viral infections, FokI polymorphism with high frequency Below the T allele are involved. Also in the present study it was observed that there was no difference between the distribution of different genotypes of the studied polymorphisms in terms of vitamin D levels.