Evaluation of TLR2,TLR4,TLR9 expression with Angiopoietin 4 proteins affection on mono nuclear cells in MS patients

Abstract

MS is an inflammatory disease in which the myelin sheaths of nerve cells in the brain and spinal cord are damaged. This injury can impair the ability of the parts of the nervous system that are responsible for communication and cause many physical signs and symptoms. TLRs are also present in the brain in addition to blood cells and tissue epithelial and endothelial cells. The reason for choosing TLR2, TLR4 is due to the great importance of these two receptors in transmitting messages at the cell surface and membrane surface. Materials & Methods: 30 MS patients referred to MS clinic of Imam Reza Hospital with moderate to severe stages were selected and blood samples were taken from patients and MACS monocytes were isolated from PBMC. Monocytes were evaluated for purity percentage by flow cytometry. Was treated with CD14 antibody. Monocytes were then cultured and stimulated with angiopoitine-4 proteins at zero, six, twelve, and twenty-four and forty-eight hours. After stimulation, cells were collected and TLR2 and TLR4 expression levels were evaluated by Real Time PCR. Results: The expression of TLR2 and TLR4 receptors decreased significantly with time course of stimulation over time, which reached its lowest value in 12 hours after stimulation. Compared with TLR2 and TLR4, the rate of reduction was the same. The performance of angiopoitine 4 was evaluated as a time course.