Blocking the growth and development of cancer cells through combintorial suppression of HIF-1α and STAT3

Abstract

Due to the fact that many factors are influential in the process of tumor formation, targeting one factor alone may not have the necessary efficiency and ability to overcome the tumor. Therefore, targeting several factors simultaneously can Be effective in controlling cancers. One of these factors is the induced factor in hypoxia (HIF-1α). Hypoxia of the environment is associated with changes in metabolism, which in turn can increase the proliferation and angiogenesis of tumors. Other factors in the development of cancer cells include stat3. Therefore, we decided to suppress cancer cells by blocking HIF-1α STAT3 molecules. Materials and Methods: In the present study, we used superparamagnetic iron oxide (SPION) nanoparticles loaded with siRNA to turn off the STAT 3 and HIF-1α genes. Also in this study, to increase the stability and improve the performance of iron oxide nanoparticles, thiol chitosan and trimethyl chitosan and hyaluronate were used to enclose them. Results: SiRNA-loaded nanoparticles coated with thiol chitosan, trimethyl chitosan and heuronate with suitable physical and chemical properties, high cell uptake and low toxicity are suitable agents for gene therapy. HIF-1α and STAT 3 are associated with significant reductions in proliferation, migration, and angiogenesis and colony formation in tumor cells.