protective effect of eugenol on histological and oxidative stress induced by torsion/detorsion in adult rat ovarian

Abstract

The current study was designed to investigate the effect of eugenol on histopathological changes and oxidative stress caused by torsion /detorsion in the ovary of adult female rats. In this study, forty-eight female Wistar rats were classified into six groups; Sham and 2 healthy group receiving 30, 60 mg/kg eugenol; ovarian torsion/detorsion; and 2 experimental groups receiving 30, 60 mg/kg eugenol. After ten days, the plasma levels of estrogen, testosterone, and some oxidative stress markers were assessed. also, the histomorphometric study was performed. A marked degenerative changes in the TD group was observed(p<0.001). The estrogen, GPX, and SOD levels were remarkably declined in the G2 group, while they were reversed to the baseline values in groups receiving eugenol. The concentration of malondialdehyde (MDA) was remarkably increased during the ischemia (p<0.001). The treatment with eugenol significantly diminished MDA levels in different groups (p<0.001). Our finding indicated that eugenol could protect the ovarian tissue against oxidative stress and tissue injury induced by torsion/detorsion