Effect of Acellular Wharton's Jelly substitution to FBS in Myeloid and Lymphoid differentiation ability of Hematopoetic Stem Cell CD34+ co-cultured with MSC

Abstract

The elemental composition of these media is complemented by animal serum, especially fetal replacing acellular Wharton's jelly with bovine fetal serum on the differentiation of hematopoietic stem cells into the erythroid lineage. Materials and methods: UCB-derived CD34+ cell were Cultured in the presence of 20 ng/ml SCF and 4.5 u/ml EPO to evaluate the effect of replacing A Cellular Wharton's jelly with FBS on early differentiation into erythroid lineage in IMDM control groups containing 10% FBS and IMDM test group comprising 10%A cellular: Wharton's Jelly. The expression of glycophorin A(CD235) on the cell membrane surface was investigated by flow cytometry technique . Results: In this study, the expression of glycophorin A (CD235) in the presence of A Cellular Wharton's Jelly on the first and 3rd days was significantly increased compared to the control group.