The effect of chemotrapuetic agents in PD-L1 expression in breast cacner cell line

Abstract

The present study on the performance of common chemotherapy drugs includes: doxorubicin, paclitaxel and docetaxel, which measured the expression of PD-L1 gene by qRT-PCR before and after the effect of Invitro chemotherapy drugs in MD231, MD468, SKBR3 cell lines, which includes various subtypes of cancer cells, including triple-negative and basal-like, and compared with the results of similar articles. Materials & Methods: First, breast cancer cells are cultured in RPMI-1640 culture medium, then the expression of PD-L1 gene in different cancer lines is measured by qRT-PCR. The growth and proliferation of cancer cells were examined by MTT test. After adding chemotherapy drugs and the required time elapsed, the culture medium was replaced with culture medium containing MTT. PD-L1 gene expression is also measured by qRT-PCR after the addition of chemotherapy drugs to various breast cancer categories.

Results: The expression of PD-L1 gene increased after 24 and 48 hours after treatment with doxorubicin, paclitaxel and docetaxel. The analysis showed that the increase in expression of this gene in response to these agents in both time periods is statistically significant.