anti-diabetic effects of Marrubium propinquum methanol extract on Streptozotocin -induced diabetic male mice

Abstract

Lamiaceae family has held its therapeutic position in herbal medicine by several known healing attributes. In traditional medicine of several nations, Marrubium plant is used to treat diabetes. In traditional Iranian medicine, Marrubium is prescribed as a tonic for mines, cardiotonic, anti fever, and in foreign applications it is used as a disinfectant. Aim: Evaluation of anti-diabetic effects of methanolic extract of Marrubium propinquum on streptozotocin-induced male diabetic mice Materials and Methods: Methanolic extract of the plant was extracted by Soxhlet method. Male mice were divided into 5 groups. The first group was a healthy control group; the second group (diabetic control group); the third group (a healthy control group receiving the extract at a dose of 100 mg / kg); The fourth group (diabetic group receiving the extract at a dose of 100 mg / kg) and the fifth group (diabetic group receiving the extract at a dose of 300 mg / kg). In order to induce diabetes in rats, 40 mg / kg streptozotocin was injected intraperitoneally to the animal 5 days after dissolution in normal saline. After confirming that the animals were diabetic, the extract was injected daily for 18 days. Before induction of diabetes and then every three days, weight and blood sugar of the animals were measured and two days before surgery, OGTT test was performed. Results: IP injection of 300 mg/kg methanolic extract of M. propinquum reduced blood sugar in diabetic rats and improved oral glucose tolerance; and reduced serum SGOT and SGPT, which were significant for doses of 100 and 300 mg / kg. Conclusion: M. propinquum methanolic extract may play a protective and therapeutic role in diabetes by increasing insulin secretion and decreasing blood sugar and MDA levels and serum SGOT and SGPT enzymes.