نمایش پرونده ساده آیتم

dc.contributor.advisorAtyabi, Fatemeh
dc.contributor.advisorGhalamfarsa, Ghasem
dc.contributor.advisorkazemi, Tohid
dc.contributor.authorIzadi, Sepideh
dc.date.accessioned2021-02-16T10:11:48Z
dc.date.available2021-02-16T10:11:48Z
dc.date.issued2020en_US
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/63683
dc.description.abstractHypoxia-inducible factor (HIF) is one of the critical components of the tumor microenvironment that is involved in tumor development. HIF-1α functionally and physically interacts with CDK1, 2, and 5 and stimulates the cell cycle progression and Cyclin-Dependent Kinase (CDK) expression. Therefore, hypoxic tumor microenvironment and CDK overexpression lead to increased cell cycle progression and tumor expansion. In the present study, we used the carboxylated graphene oxide (CGO) conjugated with trimethyl chitosan (TMC) and hyaluronate (HA) nanoparticles (NPs) loaded with HIF-1α-siRNA and Dinaciclib, the CDK inhibitor, for silencing HIF-1α and blockade of CDKs in CD44-expressing cancer cells. Since CD44 is overexpressed in many cancers, therefore, conjugation of NPs with HA can improve treatment efficacy. The results indicated that the manufactured NPs had conceivable physicochemical properties, high cellular uptake, and low toxicity. Moreover, combination therapy of cancer cells using CGO-TMC-HA NPs loaded with HIF-1α siRNA and Dinaciclib (SCH 727965) significantly suppressed the CDKs/HIF-1α and consequently, decreased the proliferation, migration, angiogenesis, and colony formation in tumor cells. These findings indicate the ability of CGO-TMC-HA NPs for dual drug/gene delivery in cancer treatment. Furthermore, the simultaneous inhibition of CDKs/HIF-1α can be considered as a novel anti-cancer treatment strategy.en_US
dc.language.isofaen_US
dc.publisherTabriz University of Medical Sciences, Faculty of Medicineen_US
dc.relation.isversionofhttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/63682
dc.subjectCarboxylated Graphene Oxideen_US
dc.subjectnanoparticleen_US
dc.subjectHypoxia inducible factor-1αen_US
dc.subjectDinacicliben_US
dc.subjectCyclin dependent kinasesen_US
dc.titleCombinatorial inhibition of hypoxia inducible factor-α (HIF-1α) and cyclin dependent kinases (CDKs) in cancer cell lines, in vitroen_US
dc.typeThesisen_US
dc.contributor.supervisorJadidi, Farhad
dc.identifier.docno609778en_US
dc.identifier.callno9778en_US
dc.description.disciplineMedical Immunologyen_US
dc.description.degreeMSc Degreeen_US


فایلهای درون آیتم

فایلهاسایزفرمتنمایش

هیچ فایل مرتبطی وجود ندارد

این آیتم در مجموعه های زیر مشاهده می شود

نمایش پرونده ساده آیتم