Performance evaluation of oxaliplatin liposomes modified with cell penetrating peptides on breast cancer cells

Abstract

Introduction: One of the new strategies to change the biological distribution of drugs is to encapsulate the drug in nanoparticles, especially liposomes. Liposomes are used as suitable carriers for hydrophilic drugs such as oxaliplatin due to their hydrophilic nucleus. Objective: Synthesis and performance evaluation of oxaliplatin liposomes modified with cell penetrating peptides on breast cancer cells (MCF-7) Methods: For the synthesis of peptide sequences, the solid phase peptide synthesis (SPPS) method was used.Then, film hydration method was used to prepare modified liposomes with CPPs. Size distribution and zeta potential of the prepared samples was assessed using zetasizer. Cytotoxicity of oxaliplatin modified liposomes with CPPs was analyzed by MTT assay.

Results: The mean volume number of the prepared liposomes was 110.5 nm and the mean drug loading was 26%. Comparison of the toxic effect of modified liposomes with CPPs containing oxaliplatin relative to the oxaliplatin showed that (RW)4 modified liposomes containing oxaliplatin relative to the oxaliplatin at concentrations of 10 and 15 μM after 48 hours 53% and 65% and after 72 hours, 63% and 69% had lethal effects, respectively. Also, R8-modified liposomes containing oxaliplatin compared to the oxaliplatin at concentrations of 10 and 15 μM after 48 hours 49% and 53%, and after 72 hours 54% and 59%, had lethal effects respectively.

Conclusion: The use of liposomes modified with cell penetrating peptides as oxaliplatin carriers increased the cell proliferation reduction in MCF-7 cell line and modified liposomes with (R-W)4 and R8 had the greatest effect (48-69%) on increasing the cytotoxicity.