Evaluation of the diagnostic value of salivary heat shock protein (HSP70) in patients with breast cancer

Abstract

Introduction: Breast cancer is one of the leading causes of death among women worldwide. Although survival rates have improved in recent years due to early detection and more effective treatments, many breast cancers are not diagnosed early. Therefore, identifying relevant and suspicious markers will lead to early detection, better prognosis, and more effective cancer treatment in the future. HSP-70 is a type of HSP that may be a proinflammatory cytokine that leads to tissue damage in this disease. Considering that all the studies have been prepared from the serum of patients with breast cancer and no study has been done on the salivary HSP-70 level in these patients, also, preparing people's saliva is morally non-invasive compared to preparing their serum. Therefore, this study was designed and performed to evaluate the diagnostic value of HSP-70 levels in the saliva of these patients. Materials and Methods: In this study, 45 patients with breast cancer referred to the chemotherapy and radiotherapy wards of Shahid Madani Hospital in Tabriz from March 1998 to August 2010 and 45 healthy individuals were evaluated. After taking saliva samples from patients and healthy individuals during the mentioned period, salivary HSP-70 was measured using ELISA method and HANGZHOU kit. Mann-Whitney test was used to compare the level of HSP-70 between healthy and sick people, as well as to compare this marker in patients with positive and negative axillary lymph node involvement. The sensitivity, specificity and diagnostic value of this protein were also evaluated through ROC curve and cut-of-point determination; the software used in this study was SPSS 25 and P value less than 0.05 was considered significant. Results: The mean age of patients were (52.13 ± 6.56 years) and the mean age of healthy individuals were (53.62 ± 8.40 years). The mean salivary HSP70 was obtained in patients (8.82 ± 15.41 ng / ml) and in healthy individuals (6.28 15 15.03 ng / ml). (0.05 p <); Also, the sub-curved area of the ROC diagram was 0.497 Conclusion: The results of this study showed that salivary HSP70 levels in patients with breast cancer were not significantly different from salivary HSP70 levels in healthy individuals and according to the ROC curve, salivary levels of this protein do not have a good diagnostic function to identify healthy and sick people.