Evalation of cell adherence and cytotoxity of dental pulp stem cells transferred on biodegradable hybride polyster-polyacrylate nanogels: as injectable scaffolds in tooth root canals

Abstract

Introduction: In reconstructive dentistry, the reconstruction of soft and hard tissue parts is considered. In this regard, the design of appropriate cell substrates that act as cell carriers and stimulus factors is of particular importance. The aim of this study is to investigate the adhesion and proliferation of oral mucosal epithelial cells in injectable mucosal scaffolds containing alginate and collagen polymers.

Materials and methods: Fibroblast cells were purchased from the National Center for Genetic and Biological Resources of Iran and then reproduced. These cells were cultured in DMEM and FBS 10% and 1xAb of penicillin streptomycin medium. Natural polymers were used to prepare the scaffold. Natural polymers of alginate, collagen and chitosan are the main constituents of scaffolds. The reproduced cells were shed on the scaffolds after the scaffold disinfection process. 100,000 Cells were placed in a plate of 6 cells on the scaffolds. For this purpose, 3 scaffold cells with cells and 3 empty scaffold cells were prepared for SEM examination on the 5th day. Surveying of Cell proliferation was performed on 1th,3rd,5th days by MTT assay as well as adhesion.

Findings:According to the results of this study , the amount of live cells in the case and control groups were similar and did not differ after 24 hours, but on 3rd and 7th days, the rate of cell proliferation in the case group was significantly higher than the control group.

Conclusion: Based on the obtained data in this study, injectable mucosal scaffolds designed for adhesive design in this project increase the rate of cell proliferation, which indicates the usefulness of these scaffolds for the repair of mucosal wounds. Therefore, these scaffolds can be studied in future studies to release drugs or substances useful for wound healing.