Evaluation of the effect of Curcumin nanoparticles on the induction of mineralization of dental pulp stem cells

Abstract

Introduction: Regenerative therapies in dental problems, especially endodontics, are rapidly developing. For this reason, induction of mineralization impact on dental pulp stem cells is a a critical factor. The aim of this study was to evaluate the biocompatibility and induction of mineralization by curcumin nanoparticles in human dental pulp stem cells. Materials and Methods: In this study, curcumin nanoparticles were successfully synthesized, then to evaluate the toxicity, in concentrations of 0.5, 1, 2.5, 5 and 10 μM at 48, 72 hours and 7 days were measured by MTT cell test and a special kit was used to determine the activity of alkaline phosphatase. Statistical analyses were performed using SPSS software version 17 . Statistical significance was determined using t-test and ANOVA.(p<0.05) Results: The results of the present study showed that nanocurcumin in most concentrations for 48 and 72 hours is not toxic to dental pulp cells and only at a concentration of 25 μM inhibition of cell growth has been done. Also, comparing the toxicity of different doses at 7 days showed that at concentrations of 2.5, 5, 10 and 25 μM, cell growth was significantly inhibited. Alkaline phosphatase activity showed a significant increase in two weeks compared to one week and also compared to the control group. (p <0.05) Conclusion: Considering the positive effects of nanocurcumin on mineralization of dental pulp stem cells and its low toxicity, this substance can be considered in planning of regenerative protocols.