| dc.description.abstract | Purpose: The population of the earth will reach nine billion in the next few decades. The use of GM products is being developed to feed this growing population. Corn, soybean, rapeseed and cotton are the most important transgenic seeds The use of transgenic products has recently been challenged. However, these products have benefits such as tolerance to environmental stresses and resistance to diseases and insects.But, limited studies have been conducted on the potential harm of using these products. In this study we also investigated the effects of transgenic oil on the tissue of rats
Methods: Eighteen Wistsr rats (Weighting 200-220 g) were included. The rats were acclimatized to the housing conditions for 1 week during which they kept on basic diet. Then thay were subdivided and randomized into 3 groups. These 3 groups (6 animals / group) were fed either the 10% GM-oil diet group or the 10% non-GM-oil diet group and Standard pellete diet group for 13 weeks. Body weight were recorded every week, and food intake was measured every 2 days. After the end of the study, blood samples were collected for measurement of biochemical and hematological parameters and Part of the texture of liver, kidney, spleen, and pancreas for examination of tissue changes.Statistical analysis was done using SPSS ( version 24). with a p ˂0.05 as the level of significance.
Results: After 13 weeks of intervention, there was no significant difference between weight and feed consumption in three groups. On the other hand, Alterations in the amounts of insulin(p=0.048), urea(p=0.001), and , GGT(p=0.03) were statistically significant in the GM soybean oil groups and its amount was increased. HGB levels (p = 0.03) in this group significantly decreased compared to the other two groups. Meanwhile, TG levels increased in the GM soybean oil group, (p = 0.006). In the consumer group of transgenic oil in the liver tissue, lesions such as severe hyperemia, moderate necrosis and biliary hyperplasia were observed. in this group, the tissue showed all the symptoms: severe hyperemia, hemorrhage, glomerulosclerosis, and the presence of hyaline cysts in the lumen of the renal vein. In the pancreatic tissue, alterations were also observed: the presence of focal inflammation cells in Exocrine section of the pancreatitis and an increase in the number of langerhans, while these changes were not observed in the other two groups. There was no change in the spleen tissue in all three groups except for mild inflammation.
Conclusion: Findings of the present study showed that consumption of transgenic oil for 90 days in rats generally caused changes in parts of liver, kidney and pancreas tissues. It has no specific effect on spleen tissue, weight and food intake. It also causes changes in some of the biochemical parameters of the blood. Further research is needed to confirm these findings | en_US |
