Evaluation of expression of BMP2 gene in warthon stem cells after facing transfection with micro RNA 424

Abstract

Introduction and Objective: Restoration of the jaw bones in congenital diseases of the cleft palate, bone injuries resulted from trauma and accidents, defects caused by malignant or benign bone lesions, which sometimes lead to the removal of a large part of the jaw is always controversial. In recent years, bone formation regimens have been reported by miRNAs shows the important role of miRNA 424 in proliferation and osteogenic differentiation. The aim of this study was to investigate the effect of miRNA 424 in laboratory on the amount of osteoporosis of Wharton Jelly Stem Cells by evaluation of expressing of BMP2 gene . Methods: Vector containing MIR424 and BMP2 genes and GFP marker was designed and synthesized on the basis of lentivirus. After confirmation of the vector, HECKT293 cells were used for packaging. The medium was collected on cells at 24, 48 and 72 hours and placed on the Wharton jelly cells. The amount of BMP2 expression were investigated by Real time PCR at 14 days. Results: Based on the results of this study, the amount of BMP2 expression rate in the presence of microRNA424 (case group) was increased by 8.04348 compared to its absence (control group).The difference was statistically significant between the two groups with P <0.05. Discussion: According to the results of this study, the BMP2 gene expression rate in the vicinity of MIR424 is constantly increasing. As a result, this vector can be used to reconstruct bone in animal models, so that the results of these studies provide the basis for gene therapy in bone reconstruction.