Preparation of Polymeric Nanoparticles Based on Polyethylene Glycol-Poly (Lactyde-Glycolide) PLGA-PEG-PLGA Containing 5-Fluorouracil and Chrysin anticancer drugs and evaluation in HT29 colon cancer cell line

Abstract

Introduction: The study of nanostructured drug delivery systems allows the development of novel platforms for the efficient transport and controlled release of drug molecules in the harsh microenvironment of diseased tissues of living systems, thus offering a wide range of functional nanoplatforms for smart application in biotechnology and nanomedicine. Polymers are widely used for drug delivery systems because of their biocompatibility and biodegradability as well as ease in the design and preparation and efficient delivery of the therapeutic active agents to the diseased tissues.

Goals: Preparation of Polymeric Nanoparticles Based on Polyethylene Glycol - Poly (Lactyde-Glycolide) PLGA-PEG-PLGA Containing 5-Fluorouracil and Chrysin anticancer drugs and evaluation in HT29 colon cancer cell line

Methods: At first synthesis of copolymer and then loading of 5-Folorouracil and chrysin into NP and the physicochemical characterization by means of Dynamic Light Scattering and Scaning Electron Microscopy. Review of drug release by HPLC. Finally the cytotoxicity and cellular internalization were evaluated by MTT assay. Results: The results showed that the encapsulation efficacy (EE%) of 5-fluorouracil was 81.3% and for chrysin 97.5%. At pH = 5.4 and temperature 40ºC the release of 5-FU was about 99% and the release rate of chrysin was 86%. At pH = 7.4 and 37 ° C the release of 5-FU was 59% and for chrysin was 55%. MTT assay showed a statistically significant concentration-dependent manner was observed at 48 hours, and nano-combinations significantly improved this effect, especially at higher concentrations. Conclusion: We could conclude that formulated targeted PNPs could be considered as a suitable drug delivery system for this two drugs. NP with appropriate percentage of drug loading can be efficient in targeted drug delivery of cancer cells.