Evaluation of glycogen content in some bengin,dysplastic and malignant oral lesions with PAS staining in patients reffered to Tabriz dentistry faculty between 2009-2019

Abstract

Introduction Oral cancer is a major cancer and although diagnostic techniques have improved during years, survival rates are still low (1). When cancer is diagnosed early, all forms of treatment show best performance (5). The aim of this study was to analyze and compare the glycogen content in dysplastic epithelium and invasive carcinoma along with other pathological conditions and normal Oral epithelium to determine whether the glycogenic content is an important marker in the histological evaluation of epithelial dysplasia. Materials and methods In this cross-sectional retrospective research, paraffin blocks related to patients diagnosed with lichen planus (17), epithelial dysplasia (7), Verrucous carcinoma (7) and SCC (12) were examined by PAS staining. The results were reported as number (percentage) or mean standard deviation for qualitative and quantitative variables respectively. One way ANOVA was used to compare the glycogen levels in the groups if the data were normal and the non-parametric equivalent of the Kruskal-Wallis test was used if the data were abnormal. SPSS version 17 was used to analyze the data and a significance level of p <0.05 was considered. Results In our study, samples of lichen planus (29.8%), dysplasia (12.4%), Verrucous carcinoma (12.3%), SCC (21.1%) and normal tissue (24.6%) were studied as control group and the overall results are: The mean of glycogen variable in the group of keratotic and benign lesions was higher than the mean in the group of malignant lesions (P-value <0.05). The mean of glycogen variable in the group of keratotic and benign lesions was lower than the mean in the control group (P-value <0.05). B The mean of glycogen variable in the malignant lesion group was lower than the mean in the control group (P-value <0.01). Conclusion In our study, the amount of glycogen in malignant lesions was significantly lower than in the control group because benign and precancerous epithelial cells consume greater amounts of energy due to increased proliferation and therefore deplete their glycogen sources that lead to a negative reaction with PAS staining.