The effect of platelet-derived microparticles on CD34+ HSC differentiation toward granulocytic lineage cells

Abstract

The ultimate therapy for numerous hematological malignancies is bone marrow transplantation, where faster granulopoiesis is sought to ensure effective and more rapid engraftment. Platelet-derived microparticles are the most abundant EVs found in plasma. miR-223, a miRNA known to be involved in HSC fate, which promotes granulopoiesis through suppressing NFI-A gene, is abundantly found in PMPs. Methods: In this study, umbilical cord blood CD34 cells were cultured in presence of FLT3-L, SCF, IL3, GCSF as the control group and in presence of 10 and 50 μg of platelet microparticles as test groups. Finally, the expression of CD markers and genes related to the granulocytic lineage were evaluated using flow cytometry and Real Time PCR. Results: Flow cytometry analysis of CD15 and CD11b indicated better granulocytic differentiation of the treated cells. qRT-PCR revealed reinforced suppression of NFI-A gene in the control group which was consistent with the markedly high expression of miR-223 found in PMPs.