Effeect of autologous platelet rich plasma on endometrial growth in frozen embryo transfer

Abstract

Implantation is a complicated process affected by several factors and referring to the healthy contact between endometrium and embryo. Endometrial acceptibility and quality of embryo are two significant primary factors in transferring embryo through IVF/ICSI. Platelet-rich plasma (PRP) intrauterine infusion is a novel approach toward treating thin endometrium and recurrent implantation failure (RIF). Thus, the present study aimed to investigate the effects of autologous PRP on endometrium growth in cycles of frozen embryo transfer (FET). Methodology: Twenty women whose embryo transfer cycle was cancelled due to improper growth of endometrium or recurrent implantation failure were investigated in the study. Participitants were selected through random population sampling and control group was same group before adminestr PRP. Blindness of the sonography operator and However, subjects did not have to be blind for research findings were objective. Findings: In the present study, 20 women aged on average 32.35± 5.89 years and with an average BMI of 27.02± 0.56 kg/m2 entered the study. Average thickness of endometrium on the 10th day of menses was 6.80 ±1.46 mm; however, 72 hours after PRP infusion, growth in average thickness was 8.09± 2 mm. the mean of average increase in endometrium thickness was 1.28 ± 0.53 mm (P=0.02). 72 hours after PRP infusion, endometrial vascularity was good in 9 subjects (45%) and was average in the remaining 11 subjects (55%). On the other hand, two weeks after transferring embryo, HCGβ was positive in 9 subjects (45%) and was negative in the remaining 11 subjects (55%). Nonetheless, sonography results were beta positive in 9 women, positive in 8 cases (88.9 %) and in one subjects, the sonography was negative regarding pregnancy. Of 8 subjects with positive sonography, 2 subjects (25%) suffered miscarriage and 3 subjects (37.5%) gave birth. And 3 of them (37.5%) are still pregnant.