Comparison of the effect of methanolic extract of Marrubium persicum and Topiramate on vincristine-induced neuropathic pain in mice.

Abstract

One of the main side effects of vincristine is neuropathic pain and there are several mechanisms (increased NMDA grade, increased stress oxidative system, increased interferon alpha and interleukin beta) in vincristine-induced neuropathy. According to the previous reports Marrubium persicum methanol extract passess antioxidants properries. Topiramate is an antagonist of NMDA receptors. Aim. The aim of the present study was to evaluate the effect of Topiramate and M. persicum methanol extract on vincristine-induced neuropathic pain in male mice. Materials and methods: seventy two mice were in weight ranges of 25 to 35 chosen into 9 groups of 8 and different doses of topiramate(25, 50, 100 mg/kg, ip) and M. persicum methanol extract (5, 10, 15mg/kg, ip) and combined doses (topiramate, 25 mg/kg, ip + M. persicum methanol extract 5 mg/kg, ip) infused for twenty days (first three consecutive days, then every four days). Vincristine was injected on the fourth day to induce neuropathic pain. Tail flick test was used to evaluate different diets for hypersensitivity. At the end of the study serum levels of Malondialdehyde (MDA) and Total antioxidants capacity (TAC) were evaluated. Results: Different doses of topiramate and M. persicum methanol extract resulted in significant effects on the reduction of vincristine-induced neuropathy especially on the 16 and 20 days (p<0.001). Topiramate (25 mg/kg, ip) plus M. persicum (5 mg/kg, ip) did not show significant decrese in neuropathic pain on the same days. Conclusion: Maybe Topiramate by NMDA receptor antagonist effect and M. persicum methanolic extract by inhibiting oxidative stress effect can be effective on the reduction of vincristine-induced neuropathic pain.