| dc.description.abstract | The aim of this study is to investigate OS markers, inflammatory factors, related microRNAs (miRNA) expression, and cytokine and transcription factors RNA expression.
Method of study: We evaluated the frequency of helper T (Th) 17 and regulatory T (Treg) cells in recurrent implantation failure (RIF) women with or without MS. miRNA expression, an inflammatory cytokine, and transcription factors were measured by Real-time PCR. The level of interleukin (IL)-1β, IL-6, IL-17, tumor necrosis factor-alpha (TNF-alpha) and chemokine (C-C motif) ligand 2 (CCL-2), and C-X-C Motif Chemokine Ligand 8 (CXCL-8) were measured by enzyme-linked immunosorbent assay (ELISA). OS markers were evaluated by spectrophotometric assay. Th17 and Treg cell frequencies were determined by flow cytometry.
Results: The expression of AP1, NF-κB, FOXP3, miRNA-21, serum or plasma level of OS markers (i.e., nitric oxide, total oxidant status, and myeloperoxidase), serum level of inflammatory factors (i.e., IL1-β, IL-6, IL-17, TNF-alpha, CXCL-8, and CCL-2), and frequency of Th17 cells were increased in RIF-MS patients in comparison to RIF women without MS (RIF-NMS) and control group. The expression of miRNA-223 and 146a, antioxidant enzymes, namely superoxide dismutase (SOD) and catalase (CAT), and frequency of Treg also declined in RIF-MS patients. | en_US |
