| dc.description.abstract | The present study aimed to determine the frequency of capsular serotypes, virulence factors and carbapenemase genes in K. pneumoniae isolates obtained from Sina Hospital, Tabriz.
Methods: Sixty-one non-duplicated K. pneumonia isolates were collected from Sina hospital, Tabriz, Iran. Clinical and demographic data of all patients were reviewed. The disk diffusion method was performed for the determination of antibiotics susceptibility pattern. Detection of capsular serotypes, carbapenemase genes, and different virulence factors were studied by Multiplex PCR.
Results: Various levels of resistance were observed to different antimicrobial agents. Of the total, 47 K. pneumoniae (n=47; 77%) were found as multidrug-resistant (MDR), and 36 (59%) were positive for Extended-Spectrum Beta-Lactamase (ESBL) production. Amongst all isolates, 59% of isolates were categorized typeable using primers for K1, K2, K5, K20, K54, and K57. The frequency of capsular serotypes was as follows: K5 (8.1%), K20 (21.3%), and K54 (29.5 %). The frequency of carbapenemase genes including blaOXA-48, blaNDM-1, blaKPC, blaVIM, and blaIMP was 48 (78.7%), 12 (19.67%), 9 (14.75%), 7 (11.47%) and 3 (4.91%) respectively. Virulence genes encountered with high prevalence of K. pneu¬moniae isolates were 95.1% entB, 93.4% uge, 91.8% ycfM and 88.5% wabG while, those with low frequency were 6.6% iroN, 4.9% cnf, 3.3% allS and 1.6% hly. | en_US |
