Evaluation of cytotoxic activity of Anthriscus nemorosa on cancerous and non- cancerous cells (MCF7, HFF)

Abstract

Introduction: Anthriscus nemorosa (M. B.) Spreng. (Apiaceae) grows in different parts of Iran including Mazandaran, Gilan, Azerbaijan. Terpenoids, phenolics, anthocyanins, podophyllotoxins and others have been identified in Anthriscus genus which have effects like analgesic, antiviral, anti inflammatory, hepatoprotective and anti platelet aggregation . Scope: The present study concern the cytotoxic activity of A. nemorosa different extracts on cancerous (MCF-7) and normal cell lines (HFFF2). Materials and Methods: Different extracts of aerial parts of A. nemorosa were prepared using Soxhelet apparatus. The cytotoxicity of samples were assessed by MTT assay on cancerous cell (MCF-7) and noncancerous cell (HFFF2) with different concentration of extracts in 24 and 48 hours. The most potent extract was fractioned and cytotoxic activity of fractions were studied, As well. Furthermore, Flow cytometry (annexin V/PI) assay has been used for detecting the mechanism of cell death in sample treated cell lines. Moreover, for clarifying volatile components n-hex extract and its 80% and 100% VLC fractions were subjected to GC-MS apparatus. Results: Results indicated that n-Hex extract and its 80% and 100% VLC fractions exhibited significant (p<0.001) inhibitory effect on the gorwth of MCF-7 cell line compare to control group. Flow cytometry analysis revealed that potent extract caused cell death through necrosis and 80% and 100% fractions showed different mechanism (such as autophagy). The major compounds which maybe were in charge of showing cytotoxic activity, were non terpenoids.

Conclusion: This study provides the evidence that in vitro cytotoxic activity of n-Hex extract and 80% and 100% VLC fractions of A. nemorosa inhibited the proliferation of MCF7 cell line via different mechanism.