| dc.description.abstract | Introduction: Insomnia is an increasing issue and it affects various aspects of human physiology including blood coagulation. Also, due to the large number of patients treated with warfarin and its importance due to the narrow spectrum of therapy, we decided to investigate the effect of sleep deprivation on coagulation factors in warfarin-treated male rats.
Materials and Methods: This research is an experimental study. The study population consisted of 40 male Wistar rats. rats were grouped: without sleep deprivation (group I), sleep deprivation (group II), warfarin-treated non sleep deprived rats (Group III), and sleep-deprived warfarin-treated rats (Group IV). Multiple platform technique was used to induce sleep deprivation. In the warfarin-treated groups, gastric gavage was administered until the end of the sleep deprivation induction protocol, with the warfarin dose of 0.1 mg/kg in the last 5 days of protocol. In groups that did not receive warfarin, normal saline was given orally by gastric gavage. Serum corticosterone levels, PT test, relative thromboplastin time (PTT), INR, factor II (prothrombin) were measured. data were compared between groups with Kruskal-Wallis post Tukey test.
Results: Serum levels of corticosterone in sleep deprivation groups (groups II and IV) were significantly higher than those without sleep deprivation groups (groups I and III) (P< 0.05). Sleep deprivation significantly decreased PT, INR, PTT values in the warfarin-treated groups (P<0.05). Serum prothrombin levels were significantly decreased under sleep deprivation (P <0.05), but in warfarin-treated rats, sleep deprivation did not decrease Serum prothrombin significantly (P <1.000). | en_US |
