The Analysis of expression level of UBE2B and CX3CR1 in sperm cell of infertile patients

Abstract

Male infertility is one of the most prevalent Health problems around the globe which affcts millions of people. Evaluation of semen sample is most common used test for evaluation of male infertility but there is a significant overlap between semen parameters of fertile and infertile men which makes the charachtrization of male infertility difficult and the significate part of male infertility is called “unexplained infertility”. So, the researchers in the field of infertility tried to find new molecular markers for better characterization and understanding of male infertility. Material and methods: In current study sperm samples of 80 infertile male were recruited. 24 samples were asthemoteratospermia (AT), 18 asthenospermia (AS) and 17 numbers were Teratospermia (TS). 21 numner of semen samples were choosen to be normospermia (NS). Using Real-time PCR technique, expression level of three genes including UBE2B, CX3CR1 and SEMG1 were evaluated. Results: The current study indicated expression level of SEMG1 gene in all studied groups (AS, TS, AT) have been significantly altered, respectively. (p=0.047, p=0.027 and p=0.000). No significant alteration was observed in expression level of CX3CR1 gene compared to the control group. Also, using Real-time PCR no expression was observed in UBE2B gene in sperm samples.