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dc.contributor.advisorBaradaran, Behzad
dc.contributor.authorFarzamifar, Parviz
dc.date.accessioned2019-12-30T10:17:13Z
dc.date.available2019-12-30T10:17:13Z
dc.date.issued2019en_US
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/61209
dc.description.abstractGastric cancer is a fatal disease characterized by the involvement of gastric tissue. Genetic changes play an important role in the development of gastric cancer. In addition to changes in the level of DNA, changes in the expression level of MicroRNAs are also important in cancer progression. Diet plays an important role in the prevention of this disease and recent studies have confirmed the role of linoleic acid (LA) in some cancers. The aim of the present study was to evaluate the effect of linoleic acid on the expression levels of MMP-2, Talin-2, VEGF-A, Angiopoietin-A2 as metastasis-dependent genes as well as mir-194, mir-106b, mir-126 and mir-30. It is in the MKN-45 cell line. Methods: In this study, MKN45 cell line of gastric adenocarcinoma cells were cultured in RPMI1640 medium, then determined by MTT assay of effective LA dose and after 24 hours treatment with LA and Docetaxel changes of gene expression. The study was compared to the control group by Real Time PCR method. To evaluate the expression of the studied genes, total RNA of the treated cells was extracted at 50 mM LA and then cDNA synthesized according to the kit instructions and then analyzed by RT-PCR. In this method, U6 and beta-actin were used as housekeeping gene to measuremicroRNA and gene expression. Docetaxel was also used as a standard drug for the treatment of gastric cancer in addition to LA. Results: Treatment of cells with LA induced changes in expression of the studied genes. Based on the results of the study, the expression of MMP-2 and Talin-2 metastasis-dependent genes was significantly increased in the treated cells compared to the untreated cells. The expression of mir-194, mir-106b, mir-126 and mir-30 was also significantly decreased in LA-treated cells. In other words, LA resulted in altered expression of genes involved in metastasis and angiogenesisen_US
dc.language.isofaen_US
dc.publisherTabriz University of Medical Sciences, Faculty of Medicineen_US
dc.subjectMMP-2en_US
dc.subjectTallin-2en_US
dc.subjectmir-194en_US
dc.subjectmir-106ben_US
dc.subjectmir-126en_US
dc.titleEvolution the Effect of linoleic acid on expression of metastatic and angiogenic molecular markers in gastric cancer cell line MKN-45en_US
dc.typeThesisen_US
dc.contributor.supervisorShirmohammadi, Masoud
dc.contributor.supervisorKazemi, Tohid
dc.identifier.docno609366en_US
dc.identifier.callno9366en_US
dc.description.disciplinemedicineen_US
dc.description.degreeM. D. Degreeen_US


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