Optimization of L-Asparaginage by isolated halophilic bacteria from centeral districts of Iran

Abstract

Halophilic bacteria have the ability to survive in saline conditions and include several group salt tolerant, moderately halophilic bacteria and extreme halophilic bacteria. These bacteria are capable of producing hydrolytic enzymes. These include L-asparaginase, which breaks the amino acid L-asparagine into two components, aspartic acid and ammonia, and is important in the treatment of cancer. Objective: In this study, the production of L-asparaginase in Halophilic bacteria was firstly isolated and then optimization of its production was investigated.

Materials and methods:

First of all, halophilc strains DAR and D6A were screened for the production of L-asparaginase. Nine different factors of culture and physical environment for L-asparaginase production were screened by plackett-burman method. Then, the effective factors for L-asparaginase production were optimized using Box-Behnken method.

Results: The results showed that the D6A and DAR 99 strains were similar to Bacillus subtilis which were capable of producing L-asparaginase. The halophic strains of DAR and D6A were capable of producing L-aspaginase enzymes, and the factors that were most effective in optimizing its production were NaCl (6.17 g / l), KH2PO4 (4.99 g / l) and aspergin for the DAR strain and glucose (1.49 g / l), NaCl (10 g / l) and asparagine (9.98 g / l) for the D6A strain. Conclusion: The results showed that Bacillus subtilis isolated from saline soils is capable of producing L-asparaginase. The factors NaCl, KH2PO4 and Aspergin for DAR and Glucose, NaCl and Aspergin for D6A have the greatest impact on the production of L-Asperginase.