Study of Antimicrobial Activity of Scaffolds Containing Chrysin In Vitro

Abstract

Introduction and goal Application of tissue engineering in the oral mucosa can be a best solution for the treatment and removal of defects or lesions of the oral mucosa (1). For achieving this goal, preparation of engineered tissue of the oral mucosa using a suitable scaffold is important which is capable of repairing damaged tissue not only by temporarily effect in the place of injury but also by adaptation to the biological environment of the host and preventing microbial growth and secondary infection(2). According to the antioxidant and anti-inflammatory properties of chrysin, the purpose of this research was to design and synthesize chrysin-containing scaffolds and to investigate their antimicrobial activity in the experimental environment in order to prevent bacterial growth in the laboratory environment. In case of obtaining acceptable primary results, it will be used to excelerate the healing of oral wounds that are susceptible to infection. Materials and methods For this purpose, The MIC of chrysin was determined with a concentration of 1000 ppm on scaffold containing synthetic polymer raw materials from caprolactone monomer in the presence of polyethylene glycol and natural polymer of gelatin and alginate. Then the scaffolds were placed in the culture containing microorganisms including Candida albicans, Enterococcus faecalis, Streptococcus mutans, and Pseudomonas aeraginosa and Staphylococcus aureus. The microbial growth inhibition zone was measured at 24 hr. For control of the scaffolds, the same raw material without chrysin was used. Results Based on the results of this study, scaffolds containing 1000 ppp MIC of chrysin extract had antibacterial activity against Enterococcus faecalis ,Psedumonas aeraginosa , Staphylococcus aureus, Acinetobacter baumanni.Scaffolds did not have any antibacterial activity against Streptococcus mutans, E.coli and Candida albicans.