Comparison of salivary MMP- 8 activity level in patient with diabetes and moderate to severe chronic generalized periodontitis referred to Tabriz dentistry faculty at 1397

Abstract

Introduction Underlying systemic diseases such as diabetes, hormonal changes or immunological deficits change the host’s response to plaque, accelerate the progression of periodontitis, and increase the severity and extent of periodontal destruction. The aim of this research was to compare the activity of 8-salivary matrix metalloproteinase in moderate to severe generalized chronic periodontitis between healthy individuals and those with diabetes type 2 referred to Tabriz Faculty of Dentistry in 2018. Materials and methods In this cross-sectional study, 90 patients were randomly divided into three groups (patients with generalized chronic periodontitis with normal blood glucose, patients with generalized chronic periodontitis with diabetes, and 30 healthy individuals as the control group) with inclusion and exclusion criteria. For saliva sampling, participants were prohibited from brushing for the past 12 hours and eating and drinking for one and a half hours prior to saliva sampling. None of the volunteers had any problems other than the subjects being examined and did not take any medication during the research. All saliva samples were stored immediately at -80 ° C and analyzed by ELISA at the appropriate time. Results In this study, 8 salivary MMP levels were significantly different in the three groups. Patients with periodontitis and diabetes had significantly higher salivary MMP-8 levels than the other groups. The control group also had the lowest amount of salivary MMP-8. Conclusion The level of activity of 8-salivary matrix metalloproteinase was significantly correlated with chronic generalized periodontitis. High levels of 8 matrix metalloproteinase in diabetic patients indicated the effect of diabetes on periodontitis.