Evaluation of antibacterial effect of mouthwash containing Glycyrrhiza glabra extract on Helicobacter pylori as invitro study

Abstract

Introduction: Helicobacter pylori infections have infected many people around the world and several drugs used to treat it cause antibiotic resistance and therefore considered as a public health problem in many parts of the world and in Iran. The aim of this study was to investigate the antimicrobial effect of mouthwash containing Glycyrrhiza glabra extract on Helicobacter pylori in vitro. Methods: In this study, bought samples of Helicobacter pylori were used. Helicobacter pylori strains isolated from specific culture media were prepared for disk diffusion processes. At the next stage, different volumes of mouthwashes containing 12.5 and 25% of Glycyrrhiza glabra extract were prepared and added to wells. The control sample was considered daily mouthwashes with Cetylpyridinium chloride and sodium fluoride. The results were reported as mean ± standard deviation. Results: In two types of mouthwash containing Glycyrrhiza glabra extract 25% and 12.5% ,the mean diameter of the inhibition zone of bacteria at all dilutions of ، ، and was higher than the mouthwashes without Glycyrrhiza glabra extract. The highest diameter of the inhibition zone related to mouthwash 25% that wasnot diluted. There was no statistically significant difference between two types of mouthwashes in the development of the inhibition zone of Helicobacter pylori (p = 0.14) Conclusion: Mouthwashes containing 25% and 12.5% of Glycyrrhiza glabra extract compared to non-extracts mouthwash resulted in a higher mean of the inhibition zone of Helicobacter pylori