Study the effect of Coenzyme Q10 on authophagy in myocardial infarction in male wistar rats

Abstract

Introduction: Autophagy is a physiological process that, through lysosomes, destroys unnecessary cell components. Coenzyme Q10 has a well-known effect on cellular metabolism processes and ATP production and also it is an important component of the electron transfer chain and plays an important role in cell,s aerobic respiration. Objective of Study: In this research the effect of coenzyme Q10 on hemodynamic parameters and autophagy factors in heart tissue in myocardial infarction caused by isoproterenol subcutaneous injection were studied. Methods and Materials: In this study male wistar rats weighting 270-300 g which kept at 25-27 ° C and the dark and brightness cycle for 12 hours were used. Water and food were freely supplied to them. For induction of myocardial infarction, isoproterenol (100 mg/kg) was injected subcutaneously for 24 hours within two days. Oral coenzyme Q10 (10 mg/kg) was received two hour before myocardial infarction induction in several doses for 24 hours within 4 days. After the anesthesia of rats, hemodynamic parameters were examined. To perform western blotting, the heart of rats was analyzed Results: With receiving coenzyme Q10, the hemodynamic parameters did not change significantly compared to the isoproterenol receving group (0.05<p). The increase in the ratio of LC3-II/LC-I and the increase in the BECLIN1 protein represent the induction of autophagy. With the intake of coenzyme Q10, The ratio of protein LC3-II/LC3-I was significaly increased compared to the isoproterenol group (0.05>p). BECLIN1 protein was significantly increased compared to the isoproterenol group (0.05>p). P62 protein did not change significantly compared to the isoproterenol receving group (0.05<p).

Conclusion: This study shows that, coenzyme Q10, has a protective effect against infraction caused by isoproterenol, This protective effect of coenzyme Q10 is associated with an irritant effect on macroatophagia. It seems that strengthening of autophagia can protect the heart from damage of myocardial infarction.