| dc.description.abstract | Various mechanisms are involved in the development of opioid dependency, which include activation of the glutamatergic and dopaminergic systems and increased the pro-inflammatory cytokines and oxidative stress incidence. Also, previous studies indicated that the antioxidant capacity and anti-inflammatory effect of natural honey.
Aim:
The aim of this study was to evaluate the effects of chronic administration of natural honey on the development of dependence to morphine in male rat.
Materials and methods:
Natural honey (NHO) was prepared from Tarom Oliya region of Zanjan city. Experiments were performed on male Wistar rats weighing 225-275 g, divided into six groups (n=8) randomly. The study groups including: the morphine group, the three groups receiving morphine with honey at doses (200,400 and 800 mg/kg, i.p), the morphine-vehicle group, and the saline group.
To creat morphine-dependency, injection of additive doses of morphine subcutaneously for 9 days were used. On the 9th day, one hour after the morning dose of morphine, naloxone (4mg/kg, i.p.) was injected and symptoms of withdrawal syndrome were counted for 60 minutes. Then, blood samples were taken for measuring TNF-α and MDA. To compare the results, one way ANOVA and Tukey test were used. P <0.05 was considered significant.
Results:
The results of this study showed that intraperitoneal injection of honey in 3 doses (200, 400 and 800 mg / kg with p <0.001) could significantly decrease the total score of the symptoms compared with the morphine-vehivle control group. NHO could significantly decrease TNF-α at dose 400 mg / kg with p >0.001 but did not affect MDA.
Conclusion:
Our results indicated that chronic administration of NHO had beneficial effects in reducing morphine withdrawal syndrome, and this effect is probably due to the anti-inflammatory effect of flavonoids in honey. | en_US |
