Determination of doxorubicin hydrochloride in whole blood samples using poly taurine-GQDs functionalized β-cyclodextrin/chitosan modified electrode

Abstract

Introduction: Nowadays anti-cancer drugs play an important role in therapy. The development of an accurate and repeatable method for determination of these drugs will improve our understanding of their role during the treatment process. In this study, a novel nano-composite used for the modification of gold electrode by simple electrodeposition approach. The prepared interface was applied for the sensing of doxorubicin hydrochloride (DOX) in human plasma samples without any pretreatment procedure. Aim: Developing an electrochemical sensor based on poly-taurine/graphene quantum dots (GQDs) functionalized β-cyclodextrin and its application for detection and determination of doxorubicin in plasma samples. Method: An ultra-thin layer (10nm) was fabricated by electrodeposition of β- cyclodextrin/poly-taurine at the bare Au electrode surface and then electropolymerization procedure was continued by electrodeposition of GQDs (2-5nm) on thin film coated electrode. scanning electron microscope (SEM) and cyclic and differential pulse voltammetric techniques were used for characterizing of final nanocomposite. The electrochemical behavior of final nanocomposite was studied using cyclic voltammetry technique. The electrochemical behavior of DOX was investigated at final composite-Au in plasma samples, using differential pulse voltammetry technique. Results: CV results indicated that the DOX is oxidized via two electrons and two protons at the optimum pH equal to 4.0 Using differential pulse voltammetry technique causes a sensitive determination of DOX in human plasma samples which was performed with wide dynamic range (0.086-3.45µM) and a limit of detection of 0.012 µM. Conclusion: The synergistic influence of poly-taurine, β-cyclodextrin, and GQDs not only remarkably enhances electroactivity, but also speeds up the electron shift between DOX and the electrode substrates. More momentously electrostatic repulsion takes place between the prepared polymeric film and DOX resulting in the signal amplification on oxidation of this drug and lowering its overpotential and thereby sensitive and selective detection of DOX in real samples.