Effects of rutoside nanophytosomes on blood sugar in streptozocin induced diabetic rats

Abstract

Introduction: Diabetes mellitus is a metabolic disorder that reduces or stops the production of insulin -whose main role is to lower blood sugar by various mechanisms- causes long-term sugar increase in the body and as a result destroys very fine veins in the body and can involve various organs of the body, such as the kidneys, the eyes and the nerves. One of the most effective herbal compounds in the control of diabetes is bioflavonoids such as rutin. The rutin is slightly absorbed from the intestine and is also degraded in the digestive system and is unstable. Therefore, in order to improve the oral bioavailability of rutin, we can use lipid nanocarriers through the form of nanophytosome. Aim: Preparation of rutin carrier nanophytosome and in vivo study of nanophytosomes on streptozotocin-induced diabetic rats Method: Phytososomes were prepared by thin layer hydration-sonication method with 1: 3 molar ratio of rutin and lecithin and produced during homogenization, sonication and cross-extrusion filtering in sizes below 100 nm. Particle size analyzing, Zeta potential and drug loading capacity of nanophytosome for the formulation were performed and Phytososome sample morphology was studied by using scanning electron microscopy and animal studies were done on rats. Results: Nanoparticles have an average size of 72 nm and zeta potential of -22 mV and a percentage load of 93.7%. In-vivo studies showed that blood glucose in diabetic rats significantly decreased after administration of rutin nanophytosomes (p˂0.05). Also, according to the results, the effect of the nanophytosomes on other biochemical parameters associated with diabetes was more than that of the free drug. Conclusion: The method used resulted in the production of particles in a nanometer size and high loading Rutin nanophytosome that were more effective in reducing blood glucose and improving biochemical parameters compared with free drug in in-vivo studies.