Analysis Methanolic Extracted Compounds from Vitex Pseudo negundo

Abstract

Introduction: The genus Vitex from Lamiaceae family comprises 250 species in world. Some of the biological activities of the species of this genus such as treatment of female hormone disorders and anti-inflammatory were attributed to the presence of flavonoids and iridoids compounds. Although new chemical drugs have highly effective and very useful for the body, but have countless side effects, this has led the medical community to turn into a herbal remedy for treating a number of diseases. Scope: This investigation was performed in order to analyse the chemical composition of Vitex pseudo negundo and evaluation its antioxidant properties. Methods and materials: Seeds of Vitex pseudo negundo were successively extracted using petroleum ether, chloroform and methanol solvents by soxhlet apparatus. Dried methanolic extract was subjected to C18 sep-pak fractionation using a step gradient of methanol-water Further purification of fractions by using preparative reversed phase HPLC yielded 2 compounds. Structure elucidation of these compounds was accomplished by using 1HNMR and 13CNMR methods. The essential oil was obtained by hydro-distillation using a Clevenger apparatus. The composition of essential oil was determined by GC_MS. Free radical scavenging activity contents of extracts were determined using DPPH radicals. Results: Phytochemical study of the methanolic extracted yielded to identification two compounds. The study on the composition of essential oil yielded 35 compounds which were mainly composed terpenoids.The methanolic extract of herbal showed moderate free radical scavenging activity. Conclusion: Overally, biological activities of this herb might be due to the presence of phenolic derivatives in the methanolic extract.