Screening of glutaminase and cellulase producing by halophilic bacteria from central districts of Iran

Abstract

Halophilic bacteria grow in a wide range of salt concentrations. According to Kouchner classification halophilic bacteria are divided in three groups: halotolerant bacteria, moderately halophilic bacteria and extreme halophilic bacteria. Halophilic bacteria can produce several enzymes such as cellulase and glutaminase which are important in industry and pharmacy. Objective: The aim of this study is isolation of halophilic bacteria producing cellulase and glutaminase from saline soils of the center of Iran. Moreover, the optimum pH and temperature for high activity and stability of the produced enzyme were identified. Materials and methods: At first, Halophilic bacteria isolated from center of Iran were cultured and then they were screened for producing of hydrolytic enzymes which include cellulase and glutaminase. Then, the best activity and stability of the produced enzymes were studied at various pH and temperatures. Results: Results showed that the isolated halophilice bacteria were able to produce hydrolytic enzymes. The halophilic bacteria which produce cellulase were belong to Bacillus. And also the halophilic bacteria produceing glutaminase were belong to Bacillus. Furthermore, the best pH and the best temperature for optimal activity of cellulase were 8 and 40 °C, respectively. Also, the best pH and the best temperature for cellulase stability were 8 and 60 ° C, respectively. Also, the best pH and the best temperature for optimal activity of glutaminase were 8 and 50 °C, respectively. Also, the best pH and the best temperature for Glutaminase stability were 8 and 60 °C, respectively.

Conclusion:

Isolated Halophilic bacteria from the deserts of central Iran have the ability to produce hydrolytic enzymes and so that it can be the good source of hydrolytic enzymes such as cellulase and glutaminase for future research and industrial work.