Anticarcinogenic effects of methanolic extract of chrozophora tinctoria on Breast cancer cell line MCF-7

Abstract

Introduction: Chrozophora tinctoria is a species from Euphorbiaceae family, naturally occurring annual plant, used as a dyeing substance and traditionally it is used for the treatment of warts and leprosy. In this study we attempt to evaluate the cytotoxic effects of chrozophora tinctoria on breast cancer MCF-7 cell line and comparing with standard chemotherapy agent, including cisplatin using MTT assay. Aim: The aim of this study was to investigate the anticarcinogenic effects of methanolic extract of chrozophora tinctoria on breast cancer cell line MCF-7. Methods: Chrozophora tinctoria were collected and the methanolic extract of the plant were prepared. The cytotoxic effects of extracts were assessed on MCF-7 and HuGu cell lines lines using different concentrations. The extract and standard chemotherapy agent (cisplatin) were used in time dependent manner to investigate the IC50. To compare the cytotoxicities, cells without being exposed to extracts were used as control. Cells were incubated with extracts and cytotoxicity was determined by the MTT assay. The experiments were repeated 3 times and IC50 values were calculated. Data collected using the software Graph Pad Prism-6, IC50 extract obtained in three different time periods. Results: According to the results, it can be seen that the plant extract does not have a significant effect on MCF-7 cancer cells in all three time intervals of 24, 48 and 72 hours. The IC50 of anti-cancer drug cisplatin in 48 and 72 hours, are respectively 22 μg / mL (73 μmol) and 4.92 μg / mL (16 μmol). Also, with increasing time, the effect of cisplatin on healthy cells of HuGu is increased, but the plant extract does not affect the healthy cells of HuGu.

Conclusion: In general, plant extract, in these concentrations, unlike anticancer cisplatin, has no inhibitory effect on MCF-7 and healthy cells of HuGu.