Show simple item record

dc.contributor.authorJodati, AR
dc.contributor.authorBabaei, H
dc.contributor.authorAzarmi, Y
dc.contributor.authorFallah, S
dc.contributor.authorGharebageri, A
dc.contributor.authorFouladi, DF
dc.contributor.authorSafaei, N
dc.date.accessioned2018-08-26T09:44:48Z
dc.date.available2018-08-26T09:44:48Z
dc.date.issued2015
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/58692
dc.description.abstractPurpose: A protective effect for estrogens against cardiovascular problems has long been known. The aim of this study was to investigate the vasorelaxant effect of 17?-Ethynylestradiol (17?-EE) on human saphenous vein. Methods: The veins were suspended horizontally between two triangular stainless steel hooks for the measurement of isometric tension in individual organ baths containing 10ml Krebs solution, at 37ط¢آ°C and gassed with carbogen under 3gr optimum tension. The effect of different concentrations of 17?-EE (2-40 ?M) on vascular tone was investigated in veins precontracted with PGF2?. Relaxation was measured after 40min and expressed as the percent decrease of initial contraction. To determine the involvement of potassium channels, endothelium, nitric oxide synthase, guanylylcyclase and prostaglandins in the vasorelaxant effect of estrogen, the veins were incubated with tetraethyl ammonium, N-nitro-L-arginine methyl ester, methylene blue or indomethacin, respectively for 20min prior to experimentation. Responses to 17?-EE were directly compared to those obtained in the same tissues in the absence of the inhibitors. Results: The mean relaxations induced by 17?-EE with concentrations of 2, 5, 10, 20 and 40?M in tissues precontracted with PGF2? were 19.8 ط¢آ±5.5%, 26.1ط¢آ±10.8%, 32.2ط¢آ±7.4%, 48.6ط¢آ±10.8%and56ط¢آ±7.6%, respectively. The results of the inhibition of potassium channels, nitric oxide synthase, guanylylcyclase, cyclooxygenase and removing endothelium in relaxation induced by 17?-EE on precontracted veins with PGF2? proved no significant differences. Conclusion: This study showed that 17?-EE has significant vasorelaxant effect on human saphenous vein in a concentration-dependent manner. This effect is probably independent of potassium channels, nitric oxide synthase, guanylylcyclase, prostaglandin synthesis and endothelium functions. ط¢آ© 2015 The Authors.
dc.language.isoEnglish
dc.relation.ispartofAdvanced Pharmaceutical Bulletin
dc.subjectethinylestradiol
dc.subjectguanylate cyclase
dc.subjectindometacin
dc.subjectmethylene blue
dc.subjectn(g) nitroarginine methyl ester
dc.subjectnitric oxide synthase
dc.subjectpotassium channel
dc.subjectprostaglandin
dc.subjectprostaglandin F2 alpha
dc.subjecttetrylammonium
dc.subjectadult
dc.subjectaged
dc.subjectArticle
dc.subjectblood vessel tone
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectin vitro study
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectsaphenous vein
dc.subjectvascular endothelium
dc.subjectvasoconstriction
dc.subjectvasodilatation
dc.titleVasorelaxant effect of 17α-ethynylestradiol on human saphenous vein
dc.typeArticle
dc.citation.volume5
dc.citation.issue1
dc.citation.spage89
dc.citation.epage96
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.5681/apb.2015.012


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record